| title: | Development of the system for identification of therapeutic targets |
|---|---|
| reg no: | ETF5999 |
| project type: | Estonian Science Foundation research grant |
| subject: |
2.7. Biotechnology, Food and Drink Technology 3.1. Basic Medicine |
| status: | accepted |
| institution: | Estonian Biocentre |
| head of project: | Mart Ustav |
| duration: | 01.01.2004 - 31.12.2007 |
| description: | We plan to develop efficient system for identification of therapeutic targets in the human and animal cells. The system will have two basic components. First, we shall have a Tandem Affinity Purification method for identification of protein complexes, which will have a set of different vectors for expression of TAP/tagged proteins. The partners interacting with the TAP tagged protein will be identified using mass/spectrometry. The second component of this system will be two/hybrid system for animal cells, where interaction between two proteins in the specific cell compartment will be identified by Fluorescence Resonance Energy Transfer. |
| project group | ||||
|---|---|---|---|---|
| no | name | institution | position | |
| 1. | Nele Jaanson | TÜMRI | teadur | |
| 2. | Meelis Kadaja | TÜMRI | doktorant | |
| 3. | Tiiu Mandel | FIT | teadur | |
| 4. | Andres Männik | FIT | teadur | |
| 5. | Mart Ustav | Estonian Biocentre | director | |