| description: |
We have identified the cellular protein, which interacts specifically with papillomavirus E2 protein in providing segregation and partitioning of the episomal ARS plasmids lacking CEN sequence in yeast cells, which carry instead of CEN the multimeric E2 protein binding sites. Such segregation partitioning is absolutely dependent on wild type E2 protein, while mutant E2 proteins are unable to mediate such function. We propose the line of experiments for detailed study of this receptor of papillomavirus genomes in mitotic cells. These studies will include the biochemical, molecular biological, cell biology studies as well as developmental aspects of this protein in assuring the biological function. We would suggest that this protein may be the target for therapeutic intervention in development the theraputic compounds. |